3/11/2024 0 Comments Corbett research rotor gene 6000![]() ![]() Much work has been done to modify these two components to enhance or attenuate the performance of CRISPR/Cas. CRISPR RNA (crRNA), which is responsible for recognition, and Cas nuclease, which is responsible for cleavage, are the core components of CRISPR/Cas. In a new development stage, how to regulate the performance of CRISPR/Cas to better meet different needs is gradually becoming the focus of research. However, as the range of applications continues to expand, the inherent performance of CRISPR/Cas alone is no longer sufficient to meet diverse needs of an increasing number of finely divided applications. The revolutionary technology of clustered regularly interspaced short palindromic repeats (CRISPR) has transformed the fields of molecular biology including gene editing, intracellular imaging, transcriptional regulation, gene therapy, molecular diagnostics, molecular biochemical circuits and more. This simple but powerful CRISPR regulation strategy without any component modification has pioneering flexibility and versatility, and will unlock the potential for deeper applications of CRISPR technology in many finely divided fields. In addition, this strategy was applied to regulate the delayed activation of Cas12a, overcoming the compatibility problem of the one-pot assay without any physical separation or external stimulation, and demonstrating great potential for fine-grained control of CRISPR. In particular, the significantly improved specificity is expected to mark advance the accuracy of molecular detection and the safety of gene editing. Unlike previous difficult and rigid regulation of core components Cas nuclease and crRNA, only a simple switch of different external RNA accessories is required to change the reaction kinetics or thermodynamics, thereby finely and almost steplessly regulating multi-performance of CRISPR/Cas12a including activity, speed, specificity, compatibility, programmability and sensitivity. Herein, we propose a CRISPR/Cas12a regulation strategy based on the powerful programmability of nucleic acid nanotechnology. As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult to cope with diverse needs in these different fields, and how to more accurately control the performance has become a key issue to develop CRISPR technology to a new stage.
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